Applying the Principles
Intolerance
Some people are less open-minded or even prejudiced against people with specific sexual preferences. Such people should not be forced to associate with or do business with those they choose not to. As with non-aggressive racism and free speech, this is the price of a free society. Not only does such prejudice reduce their customer base, but these less tolerant people may face a backlash from others who will seek to punish them through non-judicial remedies.
Bathrooms
The owner of the property can decide under what circumstances another person may use their bathroom. Property owners are entitled to control their private property, whether residential or commercial.
Gender reassignment
Parents are the proper legal guardians of their minor children. They are afforded substantial but not unlimited discretion - they have a fiduciary duty to act in the reasonable best interest of their children. Local communities must decide the age at which a parent's consent for a minor’s surgery would be a breach of their fiduciary duty.
Conclusion
All forms of consensual sexual choices between competent adults must be legal - there is no victim. Intolerance is not a breach of the Legal Principle, but such people may face informal negative consequences from the tolerant majority.